ROTOGENE (RTPCR 5X)

Feature

Specifications

Altitude

Up to 2000 m (6500 ft.)

Dimensions

Width, 37 cm (14.6 in.); Height, 28.6 cm (11.3 in.); Depth (without cables), 42 cm (16.5 in.); Depth (door open), 53.8 cm (21.2 in.)

Features

Dynamic range, 10 orders of magnitude

Heat dissipation/thermal load

Average, 0.183 kW (632 BTU/hour); Peak, 0.458 kW (1578 BTU/hour)

Humidity

1075% (noncondensing)

Kits designed for this instrument

artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit

Operating temperature

18-30C (64-86F)

Optical System

Up to 6 channels spanning UV to infra-red wavelengths; Excitation sources: High energy light-emitting diodes; Detector: Photomultiplier; Acquisition time: 4 s. The software allows to create new excitation/detection wavelength combinations

Overvoltage category

II

Place of operation

For indoor use only

Pollution level

2; Environmental class 3K2 (IEC 60721-3-3)

Power

100240 V AC, 5060 Hz, <520 VA (peak); Power consumption <60 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5a 250 V fuse

Protocols/main application on this instrument

Gene expression analysis, microRNA detection, Virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)

Samples per run (throughput)

Tubes 0.2 ml; Strip Tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; Up to 100 samples per run using a Rotor-Disc 100

Software

Rotor-Gene Q software, supplied on the installation CD provided

Storage conditions

15C to 30C (59F to 86F) in manufacturers package; Max. 75% relative humidity (noncondensing); Environmental class 1K2 (IEC 60721-3-1)

Technology

Real-time PCR cycler

Thermal performance

Temperature range, 35 to 99C; Temperature accuracy, 0.5C (type, measured 30 seconds after clock start); Temperature resolution, 0.02C (smallest programmable increment); Temperature uniformity, 0.02C; Ramp rate (peak ramp rates, air), >15C/s heating, >20C/s cooling

Transportation conditions

25C to 60C (13F to 140F) in manufacturers package; Max. 75% relative humidity (noncondensing); Environmental class 2K2 (IEC 60721-3-2)

Typical run time

40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)

Warranty

1 year on instrument; lifetime guarantee on excitation LEDs

Weight

12.5 kg (27.6 lb.), standard configuration